Coding

Part:BBa_K4144004:Design

Designed by: Boshuo Zhou   Group: iGEM22_WHU-China   (2022-10-02)


Codon-optimized Pet8p for S-adenosyl methionine transportation


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 184
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 54
    Illegal BamHI site found at 682
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is about the transmembrane protein which is expressed in mitochondrial membrane. So it should be verified to localize into membrane.


Source

The sequence is gained from Saccharomyces cerevisiae in NCBI, and is synthesized by Tsingke Biotechnology.

References